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Mortality of Mice as Affected by Variation of the X-Ray Dose and Number of Nucleated Rat Bone Marrow Cells Injected^*

N. Gengozian and T. Makinodan

( Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tenn.)

Transplantation of rat granulocyte precursors was demonstrated in mice that had received 710 r of x-rays (LD₃₀) and injection of rat bone marrow.

X-ray doses of 400–1300 r, followed by injection of rat bone marrow (140 x 10⁶ cells), resulted in a bimodal mortality curve, with maximum (100 per cent) and minimum (22 per cent) 30-day mortality occurring at 710 and 950 r, respectively, in mice that showed positive rat bone marrow transplant. Persistence of the foreign transplant among some of the 30-day survivors of these experimental groups resulted in additional deaths beyond this period.

Injection of varying amounts of rat bone marrow cells (25 x 10⁶ to 300 x 10⁶) into mice that had received 710, 800, or 950 r resulted in different mortalities and rates of mortality for each respective bone marrow dose and x-ray dose.

The results obtained in this study are in agreement with the hypothesis that injection of foreign bone marrow into x-radiated mice may result in an in vivo antigen (transplanted foreign hematopoietic tissue)-antibody (recovering immune mechanism of irradiated host) reaction, the degree (chronic or acute) and effects of which are dependent on the two variables: (a) x-ray dose applied to the host and (b) number of bone marrow cells injected.

^* Preliminary data discussed at the Biology Symposium on "Antibodies: Their Production and Mechanism of Action," Gatlinburg, Tennessee, April 8–10, 1957.

Public Health Service Research Fellow of the National Cancer Institute.

Operated by Union Carbide Nuclear Company for the U.S. Atomic Energy Commission.

Received 5/ 6/57.

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