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The Utilization of Nucleotides by the Mammal

VI. Purine Nucleotides Totally Labeled with C¹⁴ in Mice Bearing Sarcoma 180^*

Herbert Weinfeld, Paul M. Roll, Elizabeth Carroll, George Bosworth Brown and C. P. Rhoads

( Laboratories of the Sloan-Kettering Division of Cornell University Medical College, New York, N.Y.)

The incorporations into nucleic acids of adenosine-3'-phosphates, labeled in both base and sugar with C¹⁴, have been studied in the tumorbearing mouse.

When compared with data obtained in the rat, these results indicate that there was a more extensive utilization of the adenylic acid but the utilization of guanylic acid was about the same as in the rat. Guanylic acid is utilized by the mouse to a greater extent than is guanine, but the differential between the two is not so marked as that in the rat. The extensive transpurination involved during the incorporation of the guanine of guanylic acid into RNA guanine was of the order observed in the rat.

The incorporations into the tumor nucleic acids were approximately one-tenth to one-fifth of those into the visceral nucleic acids, in line with the now common observations that exogenously supplied purine derivatives are not utilized extensively in tumor tissues.

^* This investigation was supported in part by funds from the American Cancer Society upon recommendations by the Committee on Growth of the National Research Council, the National Cancer Institute, National Institutes of Health, Public Health Service (Grant No. C-471), and by the Atomic Energy Commission (Contract No. AT(30-1)-910).

Present address: Roswell Park Memorial Institute, Buffalo 3, N.Y.

Present address: Department of Biochemistry, Marquette University School of Medicine, Milwaukee, Wis.

Received 10/ 8/56.