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Human Cells in Continuous Culture

I. Derivation of Cell Strains from Esophagus, Palate, Liver, and Lung^*

( Department of Bacteriology and Immunology, University of Minnesota, Minneapolis, Minn.)

Histories of reception and propagation at the University of Minnesota, and derivation of clonally initiated sublines, are described for HeLa, Maben, liver, and conjunctival human cell strains. Origin and establishment in continuous culture of three new human strains of palate fibrocyte, esophageal epithelial cell, and liver epithelial cell are described. A clonal subline of the esophageal cell has been derived. Success in the establishment of cell strains from human surgical specimens was attributed to use of yeast extract medium and plating of cells in thin layers of plasma.

^* Aided by grants from the National Foundation for Infantile Paralysis, Inc., the National Cancer Institute, National Institutes of Health, and the American Cancer Society.

Received 6/10/57.