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Metabolic Patterns for Succinate-2-C¹⁴ in Tissues of Tumor-bearing Rats^*

William L. Nyhan and Harris Busch

( Department of Pharmacology, University of Illinois College of Medicine, Chicago 12, Ill.)

1. Metabolic patterns for succinate-2-C¹⁴ have been studied in individual tissues in vivo in rats bearing the Walker 256 carcinosarcoma at time intervals of 40 seconds to 8 minutes.

2. In the kidney the uptake of succinate, its utilization, and the outward passage of labeled compounds from the tissue were more rapid than in any of the other tissues studied. Most of the isotope found in the tissue was transferred to the amino acids, glutamate and aspartate, which contained 34 per cent of the total isotope present as early as 40 seconds.

3. In the liver the major metabolic conversion of succinate was to glucose, which contained 30 per cent of the total isotope recovered over the 3-8-minute interval.

4. In the tumor isotope was found in lactate at all time periods studied and amounted over the 3–8-minute interval to 51 per cent of the isotope of succinate that was utilized. In the presence of malonate the transfer of the isotope of succinate to lactate was almost completely blocked in the tumor.

5. These studies indicate that succinate is actively utilized by the Walker 256 carcinosarcoma in vivo. Evidence is provided for utilization of a segment of the citric acid cycle in the tumor, which could function as a source of energy and hydrogen acceptor.

^* Supported by grants-in-aid from the Jane Coffin Childs Fund and the U. S. Public Health Service (CY 2886C).

Postdoctoral Fellow of the National Foundation for Infantile Paralysis.

Received 6/11/58.