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Phosphorus Metabolism of a C3H Ascites Tumor in Vivo^*

(The Guy and Bertha Ireland Research Laboratory, Department of Biochemistry, University of North Dakota School of Medicine, Grand Forks, N.D.)

An in vivo study of the incorporation of P³² into the cellular inorganic, phospholipide, nucleic acid, and phosphoprotein compartments of C3H ascites cells from cultures of different ages has been made.

The rate of isotopic labeling into these fractions does change with culture age, and the rate of cell multiplication in vivo has been related to the relative specific activity of the nucleic acids. The logarithm of the relative specific activity of the nucleic acids has been shown to change in direct proportion to the change in the logarithm of the velocity coefficients of growth.

The rate of incorporation of P³² into the nucleic acids, phospholipides, and the nucleoproteins has been shown to be a linear function of the age of the culture in vivo.

The onset of metastasis has been associated with a reduced multiplication rate of the ascites cells and has been shown to occur some time after 42 hours of intraperitoneal growth.

^* This investigation was supported, in part, by research grants from the American Cancer Society, North Dakota Division; U.S. Atomic Energy Commission AT(11-1)-479; and American Cancer Society.

Received 10/23/57.