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Survival of the Mouse Mammary Tumor Agent (MTA) in Frozen Tissue^*

( Division of Cancer Biology, Department of Pathology, University of Minnesota Medical School Minneapolis 14, Minn.)

The mouse mammary tumor agent (MTA) was demonstrated in extracts of thawed tumor mince which had remained frozen for approximately 4.5 years.

The agent present in 10^-5 gm.-equivalents of tissue induced mammary cancer in nearly 40 per cent of the test animals, while those which received the extract at dilutions of 2 x 10^-2 to 10^-4 had a mean tumor incidence of 68 per cent.

There was no indication that the MTA had become "active" following freezing, as suggested by Gye and Mann, since the mean age for the youngest cancerous mice in the different groups was 242 days.

Viable tumor cells could not be demonstrated in the thawed suspension when tested on a small number of mice which were susceptible to grafts of the transplanted tumor.

^* Assisted by grants from the Citizens Aid Society of Minneapolis, the National Cancer Institute of the National Institutes of Health, U.S. Public Health Service, the Minnesota Division of the American Cancer Society, the American Cancer Society upon recommendation of the Committee on Growth of the National Research Council, and the Graduate School Cancer Research Fund of the University of Minnesota.

Received 2/ 5/58.