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Isotopic and Microbiological Studies of Vitamin B₁₂ Distribution in Normal and Hepatoma-bearing Rats^*

( Departments of Pathology, Internal Medicine "B," Bacteriology and Clinical Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel)

The uptake of parenterally administered B₁₂-Co⁶⁰ and the vitamin B₁₂ content were studied in rats bearing liver tumors induced by DAB.

The specific activity of B₁₂-Co⁶⁰ of the serum showed a decline during the 1st week, but was still much higher than that of either liver or tumor tissue 7 days following injection.

The B₁₂-Co⁶⁰ uptakes of the liver and tumor tissue were similar during the first 48 hours; at 7 and 12 days an increase of B₁₂-Co⁶⁰ concentration was found in the liver, but not in the tumor tissue. In contrast to the liver the B₁₂-Co⁶⁰ concentration in the kidney showed a decline with increasing time intervals following injection.

The total B₁₂ content of the tumors, as determined by microbiological assay, was about one-fifth of that of the non-neoplastic part of the liver.

^* This investigation was supported by Leukemia Research Foundation, Chicago, Ill.

Received 2/26/58.