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The Cytotoxicity of Anti-Tumor Agents for Normal Human and Animal Cells in First Tissue Culture Passage^*

( Laboratories of Microbiology, Children's Cancer Research Foundation, and Department of Pathology, Harvard Medical School, Children's Medical Center, Boston, Mass.; and Section on Experimental Therapeutics, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda 14, Md.)

Nineteen agents of known activity against one or more experimental tumors in vivo have been studied for cytotoxic activity in tissue culture against: (a) long established, (b) recently isolated, and (c) first-passage cells derived from normal human and animal tissues. The individual compounds varied widely in their cytotoxicity, but there were no regular or significant differences in the susceptibility of the various cell cultures to a given agent.

The similar response to anti-tumor agents of cell cultures derived from malignant and normal tissues was, therefore, not due to alterations in the normal cells resulting from their prolonged in vitro cultivation, but instead reflected the general cytoxoticity of these agents, evidenced even against normal cells in their first tissue culture passage, even before they have undergone extensive proliferation.

^* These studies were supported in part by research grants C-2782, C-937, and CY-3335 from the National Cancer Institute, National Institutes of Health, United States Public Health Service.

Received 2/14/58.