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Studies on the Mechanism of Action of 5-Iododeoxyuridine, and Analog of Thymidine^*

( Department of Pharmacology, Yale University School of Medicine, New Haven, Conn.)

5-Iododeoxyuridine inhibited the utilization of radioactive orotic acid, formate, and thymidine for the biosynthesis of DNA-thymine, but not of orotic acid for the biosynthesis of DNA-cytosine or of RNA-pyrimidines, by mouse Ehrlich ascites tumor cells in vitro. The analog was a competitive antagonist of thymidine in this system; however, in view of the incorporation of the iodo-compound into DNA in place of thymidine, the site of inhibition probably occurs at either the monophosphate or triphosphate level.

^* This investigation was supported by a grant (CY-3076) from the National Institutes of Health, U.S. Public Health Service.

Received 7/13/59.