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Cytochemical Fractionation of the Lettré-Ehrlich Ascites Tumor^*

( Department of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, N.Y.)

A method for cytochemical fractionation of the Lettré-Ehrlich ascites tumor has been presented. The cells were exposed to dilute Ringer solution which increased the cell size and rigidity and allowed them to be homogenized in 0.44 M sucrose with the use of the Dounce all-glass "ball and cylinder" homogenizer. Nuclear, mitochondrial, and microsome + "soluble" fractions were obtained and assayed for cytochrome oxidase and peroxidase activities. A good nuclear fraction was obtained from this tumor as shown by microscopic examination and as indicated by the low cytochrome oxidase content. The mitochondrial fraction was evaluated only by enzymatic methods. As would be expected, both cytochrome oxidase and peroxidase occurred predominantly in the mitochondrial fraction.

Spectral data indicated that the predominant pigment of the mitochondria was cytochrome c.

^* Supported in part by a grant from the Jane Coffin Childs Memorial Fund for Medical Research.

Received 8/19/59.