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Succinic Dehydrogenase Activity in HeLa Cells Infected with Newcastle Disease Virus^*

( Department of Pathology, Western Reserve University School of Medicine at Cleveland Metropolitan General Hospital [formerly City Hospital], Cleveland, Ohio)

Changes in succinic dehydrogenase activity of HeLa cells infected with Newcastle disease virus (NDV) were studied cytochemically and quantitatively. A decrease in enzymatic activity preceded morphologic changes. Succinic dehydrogenase activity decreased 56 per cent 6 hours after inoculation with the virus. There was a decrease in the number of cells in the prophase and an increase in the number of cells in the telophase of NDV-treated cells at 6 and 9 hours, but the metaphase and anaphase were unaltered. There was also a significant drop in mitotic activity at 6 hours.

With the methods employed, no evaluation of viral effect could be made in 6 hours on cells in the interphase unless the succinic dehydrogenase activity was determined.

^* This investigation was supported by USPH Service Research Grant #C-1735 and grants from the Cuyahoga Unit of the American Cancer Society and the Alice Keith Mather Fund of the Cleveland Foundation.

Received 8/14/59.