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The Phospholipides of Ehrlich Ascites Carcinoma Cells Composition and Intracellular Distribution

Donald F. H. Wallach^*, Jon Soderberg and Lee Bricker

( Department of Biological Chemistry, Harvard Medical School, and Protein Foundation, Boston, Mass.)

The phospholipide content and the intracellular distribution of lipide phosphorus of Ehrlich ascites carcinoma cells have been studied. The lipides, 58 per cent of which are phospholipides, comprise 16 per cent of the dry weight. The nuclei contain 13.7 per cent of the lipide phosphorus; mitochondria, 29 per cent; microsomes, 52.5 per cent; and supernatant, 5.4 per cent. The phospholipides include 12.5 per cent acetal phosphatides and 81.93 per cent nitrogen-containing phospholipides; 3 per cent of the lipide phosphorus is contributed by phosphatidic acid and 4–16 per cent by inositol phosphatides. The molar ratio of nitrogen to phosphorus in Ehrlich ascites carcinoma phospholipides is 1.08. Choline contributes 53 per cent of the total nitrogen; ethanolamine, 30 per cent; sphingosine, 14 per cent; serine, 7 per cent; and amino acids other than serine, 3 per cent. About 6 per cent of the ethanolamine phosphatide appears to be in peptide linkage with serine and other amino acids. The significance of these findings is discussed in the light of the micromorphologic characteristics of ascites carcinoma cells.

^* This work was supported by grants from the National Institutes of Health (C-3943), from the Kresge Foundation, and other charitable trusts; by contributions from companies interested in the alleviation or cure of human diseases.

Present address: University of Pennsylvania, College of Medicine, Philadelphia, Pa.

Received 10/ 5/59.