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The Establishment of a Cell Strain (MAC-21) from a Mucoid Adenocarcinoma of the Human Lung^*

( Cancer Research Institute, University of California Medical Center, San Francisco 22, Calif.)

A cell strain MAC-21 has been established from adult human lung tumor tissue. The tumor was identified as a well differentiated mucoid adenocarcinoma.

A relatively small clump of cells (200–300) was the source of the outgrowth that occurred about 1 month after initial cultivation in vitro. No fibroblasts were ever seen, and no alterations, transformations, or lag phase of growth were observed once the initial outgrowth appeared. The cells were epithelial in appearance and had a mean chromosome number of 79.

Viral infectivity tests, with the use of herpes simplex, adenovirus Type 8, vaccinia, and rabbit myxoma on both MAC-21 and HeLa cells gave similar results with both cell lines.

^* This investigation was supported in part by research grant CY-2713 from the Division of Research Grants, National Institutes of Health, Public Health Service; in part by grant L-37 from the American Cancer Society, Inc.; and in part by the Cancer Research Funds of the University of California.

Received 12/ 7/59.

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