This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Okada, T. A. Articles by Roberts, E. Search for Related Content PubMed PubMed Citation Articles by Okada, T. A. Articles by Roberts, E.

Cytological Analysis of Effects of Maleuric Acid on Ehrlich Ascites Tumor Cells^*

( Department of Biochemistry, Medical Research Institute, City of Hope Medical Center, Duarte, Calif.)

The injection of MA (N-carbamylmaleamic acid) into mice bearing the Ehrlich ascites tumor produced cytoplasmic blebbing and mitotic abnormalities in all phases of mitosis, inhibited the incorporation of tritiated thymidine into DNA, and prevented the progression of premitotic cells into mitosis, lesser effects being observed with smaller doses of MA. Analyses of sequential changes showed MA to be more effective intraperitoneally than subcutaneously. Cumulative effects of multiple subcutaneous doses could be demonstrated. Of a variety of substances tested, only glutathione (GSH) prevented the effects of MA. Observable effects of intraperitoneally injected MA were abolished when MA was premixed with an isomolar amount of GSH or when excess GSH was injected simultaneously or 5 minutes after MA. Fewer abnormalities were observed in the tumor cells even when GSH was given 60 minutes after MA.

^* This work was supported in part by research grant C-2568 from the National Cancer Institute, National Institutes of Health, Public Health Service.

Received 1/21/60.