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Comparison of Adenosine Triphosphatase Activity of Nuclei and Mitochondria from Mouse Mammary Adenocarcinoma^*

( Cancer Research Laboratory, Department of Hospitals and Department of Biology, New York University, New York, N.Y.)

Nuclei from a mammary adenocarcinoma and liver indigenous to the same mouse, isolated by two different methods, were shown to contain adenosine triphosphatase (ATPase) activity. The distribution of ATPase activity in subcellular portions of the transplanted mammary tumor was ascertained and found to be highest in the mitochondrial and nuclear moieties. Comparison was made between the ATPase activity of nuclei and mitochondria isolated from the same tissue with respect to the effects of pH, 2,4-dinitrophenol, ethylenediaminetetraacetate (EDTA), Mg⁺⁺ and Ca⁺⁺ cations, and water treatment of both components.

^* This investigation is part of a project, C-2565, supported by research grants from the National Cancer Institute, Department of Health, Education, and Welfare, U.S.P.H.S. Some of the data were presented at the 51st Annual Meeting of the American Association for Cancer Research, and an abstract appeared in the Proceedings.

Received 7/25/60.