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Immunohistochemical Staining Properties of the N-2-FAA Rat Hepatoma

( Department of Biochemistry Research, Roswell Park Memorial Institute, Buffalo, N.Y.)

Hepatomas induced in rats by feeding N-2-fluorenylacetamide (N-2-FAA) are not stained by fluorescein-labeled antibody to rat liver microsomes. This indicates that components of normal liver microsomes were not observed in the hepatoma. Some transplantable tumors, derived from liver tissue, the Miller hepatoma, Novikoff hepatoma, and the Simpson reticulum-cell sarcoma also do not react with the fluorescent antibody system.

The use of nonimmune fluorescent conjugate (NRG-F1) as described by Hughes et al. to differentiate tumor elements from normal components utilizing nonspecific staining technics did not show consistent lack of staining of tumor tissues in our hands.

Received 4/10/61.