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The Estradiol-17β Enzyme Systems of Human Choriocarcinoma Derived from Women

( Endocrinology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Md.)

Homogenates of seven specimens of human choriocarcinoma taken from women were assayed for three 17β-estradiol-sensitive enzyme systems: a DPN-linked dehydrogenase, a TPN-linked dehydrogenase, and an estradiol-sensitive transhydrogenase. Activities were found only in the soluble fraction of the cell precipitable at half saturation with ammonium sulfate and ranged from 1 to 50 per cent of those found in term placenta.

Received 8/11/60.