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Augmentation of 6-Aminonicotinamide Antagonism of DPN-dependent Enzymatic Systems by Diethylstilbesterol^*,

( Departments of Biochemistry and Surgery, University of Miami School of Medicine, Miami, Fla.)

The administration of testosterone, diethylstilbesterol, and combined testosterone and stilbesterol therapy had no effect on the capacity of tumor homogenates from Adenocarcinoma 755 to convert -ketoglutarate to citrate, malate to citrate, and β-hydroxybutyrate to acetoacetate. Testosterone therapy had no effect on the capacity of the niacin antagonist 6-aminonicotinamide to inhibit the conversion of -ketoglutarate to citrate, malate to citrate, and β-hydroxybutyrate to acetoacetate. The synthetic estrogen diethylstilbesterol was able to enhance markedly the capacity of the niacin antagonist 6-aminonicotinamide to inhibit the systems converting -ketoglutarate to citrate, malate to citrate, and β-hydroxybutyrate to acetoacetate. Administration of testosterone to the diethylstilbesterol-6-aminonicotinamide combination was not able to reverse the stilbesterol effect on the three enzymatic systems studied.

^* This work was supported by grants CY-2446 (C3), CY-4846, and a Senior Research Fellowship (SF-186) (L.S.D.) from the United States Public Health Service.

The following abbreviations are used:

DPN = oxidized diphosphopyridine nucleotide.

6-amino-DPN = 6-aminonicotinamide analog of DPN.

DPNH = reduced diphosphopyridine nucleotide.

Received 9/19/60.