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Establishment of the TA₃ and Ehrlich Ascites Tumors as Permanent Cell Strains in Tissue Culture^*

( Laboratory of Hygiene, Department of National Health and Welfare, Ottawa, Canada)

Two mouse ascites tumors, the Ehrlich carcinoma and the TA₃ mammary carcinoma, have been established as permanent cell strains in tissue culture. Both cell strains have been carried by serial subcultivation through more than 60 passages during a 15-month period. The cell lines have been established and propagated in synthetic medium M 150, supplemented with a low level of calf serum. When inoculated into mice after prolonged tissue culture passage, both tumor cell strains elicited ascites tumor formation within 5–8 days in 90–100 per cent of the test mice.

^* Presented at the 11th Annual Meeting of the Tissue Culture Association, Chicago, Illinois, April 6–7, 1960.

Received 9/28/60.