This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wheeler, G. P. Articles by Alexander, J. A. Search for Related Content PubMed PubMed Citation Articles by Wheeler, G. P. Articles by Alexander, J. A.

Searches for Exploitable Biochemical Differences between Normal and Cancer Cells. VI. Metabolism of Purines in Vivo^*

( Kettering-Meyer Laboratory, Southern Research Institute, Birmingham, Ala.)

Extracts of liver, intestine, blood, Adenocarcinoma 755, Sarcoma 180, Leukemia L1210, and Novikoff hepatoma from animals that had received injections of xanthine-8-C¹⁴, hypoxanthine-8-C¹⁴, adenine-8-C¹⁴, or guanine-8-C¹⁴ were examined with the aid of paper chromatography and radioautography. Uric acid and allantoin contained preponderant portions of the C¹⁴ in all the extracts following administration of xanthine-8-C¹⁴, hypoxanthine-8-C¹⁴, or guanine-8-C¹⁴. Much less radioactive uric acid and allantoin were present in the tissues following the administration of adenine-8-C¹⁴. Radioactive anabolic products as well as catabolic products were found in extracts following injection of hypoxanthine-8-C¹⁴ and adenine-8-C¹⁴. The similarities of the results for the tumors and the host tissues, the high levels of catabolic products in the blood, and the possibility that metabolic products are transported from tissue to tissue point to the likelihood that the present investigation gives information relative to the integrated metabolism of the animal as a whole rather than information relative to the capacities of individual tissues for intracellular anabolism and catabolism of purines.

^* This work was supported by the Cancer Chemotherapy National Service Center, National Cancer Institute, under the National Institutes of Health Contract No. SA-43-ph-2433, and by grants from he Charles F. Kettering Foundation and the Alfred P. Sloan Foundation.

Affiliated with Sloan-Kettering Institute for Cancer Research, New York.

Received 10/14/60.