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Heterologous Transplantation of Tissue Culture Lines^*

( Department of Pathology, Yale University School of Medicine, New Haven, Conn.)

Attempts were made to transplant neoplastic and non-neoplastic cell lines from tissue culture to animals. The subcutaneous space of hamsters and mice conditioned with x-radiation and cortisone as well as the brains and eyes of normal, unconditioned guinea pigs were used as transplantation sites. No growth of non-neoplastic cell lines was obtained in any site. Neoplastic cell lines grew poorly to produce in most instances small tumors without differentiation or organization in conditioned hosts. However, transfer of such tumors to the brains or eyes of normal guinea pigs resulted in large growths capable of serial transplantation and characterized by an organized structure and an abundant vasculature. The latter behavior contrasted sharply with the complete failure to obtain growth in normal guinea pigs with the same cell lines taken directly from tissue culture.

^* Supported by U.S.P.H.S. Grant No. B-948.

Received 12/13/60.