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Effect of Polylysine on the Leakage and Retention of Compounds by Ehrlich Ascites Tumor Cells^*

( Department of Biochemistry, University of Wisconsin, Madison, Wis.)

Washed Ehrlich ascites tumor cells were suspended in either a saline or a polylysine saline solution. The cells were centrifuged, and the supernatant above the polylysine-treated or control cells or the cell pellet was studied. Inorganic phosphate, carbohydrate, free amino acids, small peptides, potassium, and adenosine-5'-monophosphate were released from the tumor cells into the supernatant after polylysine treatment. Polylysine blocked the release of proteins and phospholipides, which passed into the suspending medium from the control cells. Antisera against Ehrlich ascites cells caused a similar change in morphology and leakage and retention of compounds from the tumor cells. This suggests that antibody-complement and polylysine may affect similar sites.

^* Published with the approval of the Director of the Wisconsin Agricultural Experimental Station. Based on a portion of a Ph.D. thesis by Steven E. Kornguth. Supported in part by the research grant (No. E-101) from the National Microbiological Institute of Health, United States Public Health Service, and the Herman Frasch Foundation.

Received 2/14/61.

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