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Electron Microscope Studies on HeLa Cell Lines Sensitive and Resistant to Actinomycin D^*

( Department of Experimental Biology, Roswell Park Memorial Institute, Buffalo, New York)

The fine structure of stock HeLa cells has been examined, and several new details have been described. Certain specific structural alterations induced by actinomycin D, a cytotoxic antibiotic, have been noted. These alterations included cytoplasmic blebbing and a fragmentation of the nucleoli accompanied by a pronounced loss in their osmiophilia. HeLa cells made resistant to actinomycin D did not display these structural changes. The findings are discussed in the light of histochemical and biochemical evidence which indicates that actinomycin D inhibits RNA and protein synthesis.

^* Supported in part by research grant #DRG-527 from the Damon Runyon Memorial Fund for Cancer Research.

Received 3/ 9/61.

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