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Tissue Culture Studies on Human Leukemia and Malignant Lymphoma

( Section of Virology and Electron Microscopy, Section of Hematology, and Department of Medicine, University of Texas M. D. Anderson Hospital and Tumor Institute; and Department of Microbiology, Baylor University College of Medicine, Texas Medical Center, Houston, Texas)

A study was made of long-term cultures of cells from lymph nodes of 72 patients with leukemia and lymphoma. This study comprised eleven cases of lymphatic, two cases of monocytic, and three cases of granulocytic leukemia, 30 cases of malignant lymphoma, and 26 cases of Hodgkin's disease. Cells from lymph nodes of seventeen patients apparently free from leukemia were also studied. Cells of the cultures derived from approximately 50 per cent of the cases of leukemia, lymphoma, and Hodgkin's disease showed a similar sequence of changes leading eventually to the death of the cultures. These changes consisted of alterations in the perinuclear zone and the occurrence of cytoplasmic inclusions suggestive of the presence of a virus. These changes were not seen in the cells of cultures derived from nonleukemic lymph nodes. The relationship of these changes to the origin of the different types of leukemia and lymphoma is discussed.

Received 7/12/61.