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A Comparison of the Respiratory Inhibitions Induced by D-Glucose and 2-Deoxy-D-Glucose in Ehrlich Ascites Carcinoma Cells^*

Kenneth H. Ibsen, Elmon L. Coe and Ralph W. McKee

( Department of Physiological Chemistry, University of California Medical Center, Los Angeles, California)

Several aspects of the effects of D-glucose and 2-deoxy-D-glucose on respiratory metabolism of the Ehrlich ascites carinoma cells are compared. After a brief stimulatory period which was longer for deoxyglucose than glucose, both compounds inhibited the oxygen consumption rate to a similar extent. Glucose-induced inhibition was released after all the glucose was utilized, but deoxyglucose-induced inhibition was unaltered at a time corresponding to the marked slowing of phosphorylation. Final release from inhibition occurred only after an extended period of respiration. Dinitrophenol initially released the respiratory inhibitions of both glucose and deoxyglucose but then inhibited the rate of oxygen consumption, particularly in the presence of deoxyglucose. A much higher concentration of deoxyglucose than of glucose was needed to induce a respiratory inhibition. Both compounds initially reduced the ATP level. This reduction was transitory in the presence of glucose and more prolonged in the presence of deoxyglucose.

^* This work was supported by a grant-in-aid (No. C-2006) from the National Cancer Institute, United States Public Health Service; and by a grant-in-aid from the Cancer Research Coordinating Committee. University of California.

This material was taken in part from a doctoral dissertation submitted to the University of California by K. H. Ibsen, Public Health Service Research Fellow of the National Institutes of Health, January, 1959.

Present address, Department of Biochemistry, Northwestern University, School of Medicine, Chicago, Illinois.

Received 7/31/61.

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