This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hoelzl Wallach, D. F. Articles by Ullrey, D. Search for Related Content PubMed PubMed Citation Articles by Hoelzl Wallach, D. F. Articles by Ullrey, D.

The Hydrolysis of ATP and Related Nucleotides by Ehrlich Ascites Carcinoma Cells^*

( Department of Biological Chemistry, Harvard Medical School, and Protein Foundation, Boston, Mass.)

The ATPase of Ehrlich ascites carcinoma cells has been examined. The evidence presented indicates the following: (a) ATP hydrolysis occurs at the cell surface, (b) the cell membrane goes into the microsomal fraction upon cell rupture and differential centrifugation, (c) ATP hydrolysis depends upon the nature and concentration of external activator ions, (d) hydrolysis is not limited to ATP; other nucleoside triphosphates are attacked with equal vigor, and ADP and AMP are also hydrolyzed, (e) ATP hydrolysis appears to proceed in stepwise fashion via ADP and AMP to adenine and inorganic phosphorus, (f) ATP, but not ADP or ITP hydrolysis, is stimulated by 2,4-dinitrophenol, but this effect is reversed by glucose.

^* This work was supported by a grant from the National Institutes of Health (C-3943).

Received 8/21/61.

This article has been cited by other articles:

				J. W. DePierre and M. L. Karnovsky Ecto-Enzyme of Granulocytes: 5'-Nucleotidase Science, March 15, 1974; 183(4129): 1096 - 1098. [Abstract] [PDF]