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Serial Heterotransplantation of Human Adenocarcinoma #1 in the Cheek Pouch of Unconditioned Adult Syrian Hamsters^*

( Department of Epidemiology and Microbiology, University of Pittsburgh, Graduate School of Public Health, Pittsburgh, Pa.)

This report describes studies which established that Toolan's human adenocarcinoma #1 (H.Ad. #1), a human tumor transplantable in conditioned laboratory animals, could be serially transplanted in cheek pouches of normal unconditioned hamsters and under these conditions for more than 60 serial passages has retained proliferating human tumor tissue. (Tumor passaged in normal hamster is herein called H.Ad. #1U.) The results are as follows: (a) histologically, H.Ad. #1U closely resembles early generations of H.Ad. #1 in conditioned animals; (b) human hemagglutinins were produced by guinea pigs immunized with either H.Ad. #1U tumor or cell culture line; (c) human cellular antigen was demonstrated in H.Ad. #1U tumor cells and cell culture lines by two independent indirect fluorescent antibody systems; (d) the karyologic state of H.Ad. #1U tumor apparently ranges from sub-diploid human to polyploid human. Some potential factors which may account for the apparent indefinite passage-ability of this tumor in unconditioned hamsters are presented.

^* This investigation was supported by research grants No. CY-4089 and C-4574 from the National Cancer Institute, National Institutes of Health, United States Public Health Service.

Received 8/ 4/61.