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Antibodies to 1,2-Naphthoquinone^*

Robert Ollodart and Noel R. Rose

( Departments of Bacteriology and Immunology, and of Surgery, University of Buffalo, Buffalo, N.Y.)

We have prepared an antibody against 1,2-naphthoquinone. This was accomplished by conjugating the quinone to bovine gamma-globulin. The conjugation product showed a visible light absorption peak of 445 mµ. The globulin-quinone antigen was used to produce rabbit antibodies shown to be specific for 1,2-naphthoquinone. The antibodies could be demonstrated by tanned cell hemagglutination and by passive cutaneous anaphylaxis. Quinone conjugated to human serum served as antigen in the former test. In passive cutaneous anaphylaxis, the quinone itself provoked the reaction. However, experimental evidence suggests an in vivo conjugation of the compound with guinea pig serum. The extent of cross-reaction with related naphthoquinone derivatives could be determined quantitatively by inhibition of hemagglutination. The possible value of this type of serological reagent for studying the process of carcinogenesis is discussed.

^* Supported in part by P.H.S. research grant C-5203 from the National Cancer Institute, National Institutes of Health, Public Health Service.

Postdoctoral Research Fellow of the American Cancer Society. At present, Resident in Surgery, Buffalo General Hospital.

Received 11/ 2/61.