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Studies on the Chemicals Required To Induce Alterations of Morphology and Growth Potential in Human Cell Culture^*

( Reuben Peterson Memorial Research Laboratory, Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan)

An analysis of a trypsin-antibiotic solution to determine which chemicals were required for the prompt and irreversible alteration of fibroblastic-like cells to epithelioid forms indicated that trypsin in Puck's saline A, mycostatin, neomycin, and ten essential amino acids were involved in the "transformation" process. The removal of any one of the thirteen ingredients or the addition of calcium and/or magnesium ions prevented the alteration phenomenon.

The optimum concentrations of the essential chemicals were determined.

A direct relationship was found to exist between the concentration of trypsin which induced cell alteration and the concentrations of calcium and magnesium ions which inhibited the conversion process.

The results are discussed with reference to the possible origins of the alterable fibroblastic-like cells, as well as the possible mechanisms responsible for the alteration phenomenon.

^* This work was supported by grant C-3112 from the National Institutes of Health, U.S.P.H.S.

Presented, in part, before the American Society for Microbiology, Chicago, Illinois, April 1961.

Received 12/ 8/61.