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The Preparation and Purification of Tritiated Carcinogenic Hydrocarbons^*

Beppino C. Giovanella, C. W. Abell and Charles Heidelberger

( McArdle Memorial Laboratory, Medical School, University of Wisconsin, Madison, Wisconsin)

Methods have been described for the preparation of tritiated carcinogenic polycyclic aromatic hydrocarbons of high specific activity by the Wilzbach method. A large number of highly radioactive impurities were produced during the exposure to tritium gas, and extensive purification was required. This was achieved by column chromatography followed by various paper chromatographic systems with some novel modifications. The following compounds were labeled by this method: 10-methyl-1,2-benzanthracene, 3-methyl-1,2-benzanthracene, 4-methyl-1,2-benzanthracene, 3-fluoro-10-methyl-1,2-benzanthracene, 4-fluoro-10-methyl-1,2-benzanthracene, 1,2,5,6-dibenzanthracene, 1,2,3,4-dibenzanthracene, 9,10-dimethyl-1,2,5,6-dibenzanthracene, 3,4-benzpyrene, 3-methylcholanthrene, 9,10-dimethyl-1,2-benzanthracene, anthanthrene, and 2-phenylphenanthrene-3,2'-dialdehyde.

^* This work was supported in part by grants C-1132 and CRTY-5002, from the National Cancer Institute, National Institutes of Health, Public Health Service.

American Cancer Society Professor of Oncology.

Received 3/23/62.