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Transformation of Chick Embryo Cells in Culture by Rous Sarcoma Virus—Cytochemical Studies^*

( Division of Biophysics, Sloan-Kettering Institute for Cancer Research, Sloan-Kettering Division of Cornell University Medical College, New York, New York)

Clusters of morphologically transformed cells (foci) resulting from infection of secondary cultures of chick embryo cells by Rous sarcoma virus (RSV) were compared with fibroblast-like cells outside the focal areas with the use of several cytochemical procedures. Transformed cells yielded greater precipitation of the dinitroformazan in a test involving the lactic acid dehydrogenase, NBT-reductase complex. Intracytoplasmic fluorescent material was observed in these cells following treatment with fluorescein-labeled anti-RSV serum. Differences were observed in the utilization of labeled precursors for nucleic acid synthesis following short exposures to uridine-H³ and thymidine-H³.

^* Supported by grants from the National Institutes of Health (C-3811) and the U.S. Atomic Energy Commission (AT[30-1]-910).

Received 4/30/62.