This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klein, M. Search for Related Content PubMed PubMed Citation Articles by Klein, M.

Susceptibility of Strain B6AF₁/J Hybrid Infant Mice to Tumorigenesis with 1,2-Benzanthracene, Deoxycholic Acid, and 3-Methylcholanthrene^*

Michael Klein

( Department of Anatomy, University of Tennessee Medical Units, Memphis, Tennessee)

Male mice belonging to strain B6AF₁/J were given fifteen injections by stomach tube of 1,2-benzanthracene, deoxycholic acid, or 3-methylcholanthrene, each suspended in methocel-Aerosol O.T. Injections were started on the 7th–8th day post partum. Control mice received no treatment, or methocel-Aerosol O.T. alone. Some of the mice were killed at median ages of from 340 to 444 days, others at median ages of 547–600 days.

Many of the mice exposed to benzanthracene had pulmonary adenomas and hepatomas at autopsy. On the other hand, the mice treated with benzanthracene and killed at a median age of 547 days all bore hepatomas. In addition, almost all had pulmonary adenomas. This demonstrates that benzanthracene was highly carcinogenic when treatment with this agent was instituted during infancy. This conclusion is reinforced by the the observation of numerous tumors in the lungs and livers of mice that received only two doses of benzanthracene during infancy. Although occasional pulmonary adenomas and hepatomas were found among the mice exposed repeatedly to deoxycholic acid, a comparison of tumor yields between these mice and the untreated controls, or the mice treated with methocel-Aerosol O.T. only, indicated a lack of carcinogenic activity for deoxycholic acid. Many pulmonary adenomas, hepatomas, and forestomach papillomas were noted in the mice exposed to methylcholanthrene. Several of the mice bore lymphocytic neoplasms, and others developed adenocarcinoma of the large intestine. A more potent carcinogenic effect was produced by repeated treatment with methylcholanthrene than with benzanthracene.

^* Supported by research grant CA-04097-05 from the National Cancer Institute, National Institutes of Health, U.S. Public Health Service.

Present address: Bio-Assay Section, Carcinogenesis Studies Branch of the National Cancer Institute, Bethesda 14, Maryland.

Received 7/13/63.

This article has been cited by other articles:

				S. R. Ostrowski, S. Wilbur, C.-H. S. J. Chou, H. R. Pohl, Y.-W. Stevens, P. M. Allred, N. Roney, M. Fay, and C. A. Tylenda Agency for Toxic Substances and Disease Registry's 1997 priority list of hazardous substances. Latent effects--carcinogenesis, neurotoxicology, and developmental deficits in humans and animals Toxicology and Industrial Health, August 1, 1999; 15(7): 602 - 644. [Abstract] [PDF]