This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Irving, C. C. Articles by Larson, D. M. Search for Related Content PubMed PubMed Citation Articles by Irving, C. C. Articles by Larson, D. M.

Evaluation of the Carcinogenicity of Aminofluorenols by Implantation into the Bladder of the Mouse^*

C. C. Irving, H. R. Gutmann and D. M. Larson

( Laboratory for Cancer Research, Veterans Administration Hospital, and the Department of Physiological Chemistry, University of Minnesota, Minneapolis)

To test the hypothesis that o-hydroxy amines are proximate agents in chemical carcinogenesis paraffin pellets containing the hydrochlorides of 2-amino-1-fluorenol, 2-amino-3-fluorenol, 2-amino-5-fluorenol, 2-amino-7-fluorenol, 2-fluorenamine, and 1-amino-2-naphthol were implanted into the urinary bladders of Swiss female mice. Paraffin pellets alone were implanted as controls. Histological examination of the bladders 40 weeks after implantation showed no evidence of carcinoma. Inflammatory changes, squamous metaplasia, and epithelial hyperplasia were noted with equal frequency in the control and experimental animals. The stability of implanted 2-amino-1-fluorenol-1-C¹⁴ hydrochloride and 1-amino-2-naphthol-H³ hydrochloride and the elution of these compounds from the pellets were examined by radioactive tracer methods. Implanted 2-amino-1-fluorenol-1-C¹⁴ hydrochloride was stable, whereas 1-amino-2-naphthol-H³ hydrochloride decomposed soon after implantation. The severe irritation of the mouse bladder by paraffin raises the question whether paraffin is a suitable vehicle in the bladder implantation test.

^* Supported in part by a grant (C-2571) from the National Cancer Institute, U.S. Public Health Service.

Present address: Radioisotope Service, Veterans Administration Medical Teaching Group Hospital, Memphis, Tennessee.

Present address: Department of Pathology, St. Mary's Hospital, Duluth, Minnesota.

Received 6/27/63.