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Glucose Supply by the Living Host and Glycolysis of Yoshida Ascites Hepatoma in Vivo^*

U. Del Monte and C. B. Rossi

( Institutes of General Pathology, Universities of Messina and Milan, and C.N.R. Center for Research in Cell Pathology, University of Milan, Italy)

Glucose supply by the host and glycolysis of Yoshida ascites hepatoma in vivo have been studied by measuring glucose penetration into, and lactate outflux from, the ascitic fluid in rats in which the ascites tumor had been replaced by cell-free ascitic fluid.

In ascitic fluid glucose concentration was extremely low, whereas lactate level was higher than in blood. The blood lactate level was higher in rats bearing ascites hepatoma than in normal rats.

Lactate outflux from the ascitic fluid was of the same order of magnitude as glucose influx, suggesting that almost all glucose made available to the ascites cells was converted to lactate. When the ascitic fluid was supplemented with an extra amount of glucose, there was a marked increase of both glucose utilization and lactate formation. It was concluded that when the ascites tumor had reached a certain size its cells were subjected to a glucose shortage.

^* Part of this work was presented at the 7th National Congress of the Italian Pathological Society, Bologna, May 16–18, 1961.

Permanent address: Institute of General Pathology, University of Firenze, Firenze, Italy.

Received 8/ 6/62.