This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Terner, C. Articles by Korsh, G. Search for Related Content PubMed PubMed Citation Articles by Terner, C. Articles by Korsh, G.

The Biosynthesis of Glycerides in Homogenates of Normal Lactating Mammary Gland and of Mammary Tumor Tissue of the Mouse^*

( Department of Biology, Biological Science Center, Boston University, Boston, Massachusetts)

A study of the biosynthesis of glycerides in lactating mammary gland and in a spontaneous tumor (DBA/2JAX) of the mouse is reported. Homogenates of both tissues, supplemented with yeast hexokinase to ensure maximum rates of glycolysis, were incubated under aerobic conditions with glucose-C¹⁴ (randomly labeled). Diglycerides and triglycerides labeled exclusively in the glycerol portion were formed at more rapid rates in tumor than in normal tissue. In view of the limited capacity of neoplastic tissue to synthesize fatty acids from precursors such as acetate (17), its high capacity to esterify preformed fatty acids may be an important factor in its economy. On incubation with mevalonate-2-C¹⁴ certain fractions of the fatty acids of tumor, but not of normal mammary tissue, were found to be labeled; cholesterol was, however, not radioactive in either tissue preparation.

^* This investigation was supported by Public Health Service Research Grant No. CA 04995, from the National Cancer Institute; and by Grant No. 13,600, from the National Science Foundation.

Abbreviations used: ATP, adenosine triphosphate; NAD, nicotinamide adenine dinucleotide; NADP, nicotinamide adenine dinucleotide phosphate.

Received 12/31/62.