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Sarcomycin Inhibition of Deoxyribonucleic Acid Synthesis in Ehrlich Ascites Carcinoma Cells

( McGill-Montreal General Hospital Research Institute, Montreal, Canada)

The effects of sarcomycin on the metabolism of nucleotides and nucleic acids in Ehrlich ascites carcinoma cells have been studied. Sarcomycin, at a concentration of 100 µg/ml or higher, inhibited the rate of deoxyribonucleic acid synthesis by over 90 per cent. However, only 20 per cent inhibition of ribonucleic acid synthesis was observed at a sarcomycin concentration of 100 µg/ml. Almost no inhibition of adenine-8-C¹⁴ incorporation into acid-soluble nucleotides was observed with sarcomycin up to a concentration of 200 µg/ml.

The sarcomycin inhibition of deoxyribonucleic acid synthesis was prevented by the addition of boiled liver extract or of glutathione or cysteine. Sarcomycin was inactivated nonenzymatically by glutathione.

It was tentatively concluded that the site of inhibition of DNA synthesis by sarcomycin is DNA polymerase, probably at a thiol group.

Received 4/29/63.