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Uptake of Miracil D by Cells of Sensitive and Miracil D-resistant Lines of Mouse Leukemia L1210^*

( Department of Biochemistry and Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, New York, New York, and Department of Biology, Brown University, Providence, Rhode Island)

With the aid of a spectrophotometric method of assay for Miracil D, a comparison was made of the ability of cells of the parent sensitive line and of a Miracil D-resistant subline of leukemia L1210 to remove the drug from the suspending medium during incubation in vitro.

The uptake of Miracil D by the cells was proportional to the initial concentration of drug per cell in the suspending medium and to the length of the incubation period. The incubation temperature was varied between 0° and 37° C. without affecting the extent of drug uptake by the cells; this suggests that this process is nonenzymatic.

There was no difference, under these various conditions, between the sensitive and Miracil D-resistant cells in their permeability to this agent. This finding eliminates one of the possible mechanisms of resistance of this tumor to Miracil D.

^* This manuscript is based on the dissertation presented by Mrs. Brindle to the Faculty of the Graduate School of Arts and Science of New York University in partial fulfillment of the requirements for the degree of Master of Science, April, 1960. This work was supported in part by United States Public Health Service Research Grant CA 02332 from the National Cancer Institute and by the Alma Toorock Memorial for Cancer Research.

Received 5/22/64.