This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Schuurmans, D. M. Articles by Olson, B. H. Search for Related Content PubMed PubMed Citation Articles by Schuurmans, D. M. Articles by Olson, B. H.

A Bioautographic System Employing Mammalian Cell Strains, and Its Application to Antitumor Antibiotics^*

( Michigan Department of Health, Division of Laboratories, Lansing, Michigan)

A bioautographic method is described which employs Sarcoma 180 (Foley) or Detroit 6 (Stulberg). The choice was based on the sensitivity of each cell strain to the compounds being tested. The cells were grown in shaker flask cultures and then suspended throughout an agar layer. Paper strips bearing antitumor antibiotics were placed on the agar surface for 1 hour, followed by incubation for 2 days at 36°C. The location of the test compounds was determined by measuring the inhibition of cellular dehydrogenase activity. Resazurin was used for this purpose. The R_F in four solvent systems and the electrophoretic migration in two buffer systems are presented for 32 antitumor antibiotics.

^* This study was performed under Contract No. SA-43-ph-1921, Cancer Chemotherapy National Service Center, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.

Received 7/15/63.