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Metabolism of Ascites Tumor Cells

III. Effect of 2-Deoxyglucose Phosphorylation on Phosphorus Metabolism^*

R. B. McComb and W. D. Yushok

( Division of Cancer Biochemistry, Biochemical Research Foundation, Newark, Delaware)

The phosphorylation of 2-deoxyglucose by respiring Krebs-2 ascites tumor cells and the effect of 2-deoxyglucose metabolism on cell orthophosphate, acid-labile phosphate, and adenine nucleotide-phosphate in these cells was investigated. In the presence of 2.0 mM orthophosphate 2-deoxyglucose (2DG) was taken up initially very rapidly, 5.7 µmoles/min/ml of cells, but uptake fell off to a steady state rate of less than 5 per cent of this rate after 20 minutes. Most of the 2-deoxyglucose taken up by the cells was accounted for as 2-deoxyglucose-6-phosphate under these conditions. Orthophosphate was also taken up by the cells incubated with 2-deoxyglucose at a rate which approximated that of steady-state 2-deoxyglucose uptake. In a medium to which no orthophosphate had been added, over-all 2-deoxyglucose uptake was diminished, and the orthophosphate content of cell suspensions was found to have fallen to half its original value within 30 seconds after addition of 2-deoxyglucose. Cellular acid-labile phosphate and adenosine high-energy phosphate were also found to have fallen to 46 and 23 per cent of the original concentration of each within 2 minutes after the addition of 2-deoxyglucose. There was a net loss of 65 per cent cellular adenine nucleotides within 12 minutes after addition of 2-deoxyglucose.

^* This work has been supported in part by Public Health Service Research grant C-5118 from the National Cancer Institute.

Present address: Biochemical Research Laboratory, Hartford Hospital, Hartford 15, Connecticut.

Received 3/ 6/63.

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