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Localizing Properties of Anti-Rat Hepatoma Antibodies in Vivo^*

Masahisa Kyogoku, Yasuo Yagi, Jakob Planinsek, Joseph Bernecky and David Pressman

( Department of Biochemistry Research, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York)

The localizing properties of the antibodies against the stroma sediments of N-2-fluorenylacetamide-induced hepatoma and normal liver were compared in tumor and normal rats. Radioautography with I¹²⁵-labeled antibodies and the fluorescent antibody technic were used.

When the globulin fractions of both antisera were injected directly into the tumor-bearing rat as well as the normal rat, antibody localized on the walls of sinusoids and in connective tissue parts of tumor as well as in normal liver. No difference has been found between the localization properties of both sera.

However, after passage through normal rat there were some differences in the localization pattern of these two antisera in the tumor. The anti-hepatoma antibody still localized specifically in the dense connective tissue, but no such preferential localization was observed with the anti-normal liver serum. There was no specific localization on the wall of sinusoid-like vascular channels with either serum, a condition clearly observed when the original globulin was used.

The possible reason for this difference was discussed, and the usefulness of the combination of I¹²⁵-radioautography and fluorescent antibody technic for the detection of the site of localization of antibody at a cellular level is stressed.

^* Supported in part by the American Cancer Society, Grant T-242, and the Atomic Energy Commission, Contract AT(30-1) 2651.

Fulbright Research Scholar on leave of absence from the Department of Pathology, Kyoto University Medical School, Kyoto, Japan.

Received 8/ 2/63.