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Preparation of Purified I¹³¹-labeled Antibody Which Reacts with Human Fibrin. Preliminary Tracer Studies on Tumor Patients^*

( Department of Radiation Biology, University of Rochester, School of Medicine and Dentistry, Rochester, New York)

Technics are described for preparing, from sera of rabbits immunized with human fibrinogen, I¹³¹-labeled antibody reacting with fibrin and suitable for diagnostic use as an intravenously administered tracer in human patients. The final purified sterile preparations contained I¹³¹ label at a level of approximately 10 mc I¹³¹/mg rabbit antibody protein. The labeled preparation almost quantitatively bound to freshly formed human fibrin in the presence of the serum proteins but did not bind to rabbit fibrin.

Twenty-eight human patients have been given injections intravenously of this type of preparation with no untoward effects. Scintillation scanning of the head area of those patients with known or suspected brain tumors indicated that preparations of this type may be useful for diagnosis and delineation of those lesions.

Possibilities of increasing the effective preferential localization of this radioactive material in tumors for diagnostic or therapeutic purposes by the concurrent use of epsilon amino caproic acid, or other inhibitors of plasminogen activation or plasmin activity, are discussed.

^* This paper is based on work performed under contract with the United States Atomic Energy Commission at the University of Rochester Atomic Energy Project, Rochester, New York.

Received 8/12/63.

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