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The Stimulatory Effect of 3-Methylcholanthrene on Benzpyrene Hydroxylase Activity in Several Rat Tissues: Inhibition by Actinomycin D and Puromycin^*

( Diagnostic Research Branch, National Cancer Institute, Bethesda, Maryland)

The presence of benzpyrene hydroxylase activity in the small intestine, lung, and kidney of the normal rat has been shown by quantitative assay and compared with the level of activity in the liver. The intraperitoneal injection of methylcholanthrene increased the level of activity found in each of the four tissues examined. The stimulation by methylcholanthrene was inhibited by the simultaneous administration of puromycin of actinomycin D, suggesting that the changes in enzyme level are dependent on protein and RNA synthesis in these tissues. Possible implications to a theory of carcinogenesis are discussed.

^* A preliminary report of some of the work described has been presented at the 54th Annual Meeting of the American Association for Cancer Research, May 23–25, 1963, and in reference 14.

Received 10/21/63.

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