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Phosphatides in Mouse Epidermis Undergoing Normal and Abnormal Growth Changes^*

( Department of Biochemistry Research, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York)

Silicic acid chromatography was employed to determine the distribution of the phosphatides in mouse epidermis undergoing normal growth changes induced by the hair growth cycle. These included epidermis removed 4, 12, and 22 days after plucking of the hair in both sexes of mice. Furthermore, the distribution of phosphatides was determined in mouse epidermis made hyperplastic by three, eighteen, and 24 applications of methylcholanthrene on alternate days for 1, 6, and 8 weeks, respectively, and in carcinogen-induced squamous-cell carcinomas. There were no significant differences in the per cent distribution of the various classes of phosphatides eluted from silicic acid between epidermis undergoing normal and that with cancerous growth changes, in spite of the fact that the per cent of total phosphatides in the various examined carcinomas and epidermis varied considerably. Chromatography of the various fractions on silicic acid-impregnated paper indicated the presence of the following phosphatides in normal and treated epidermis in about the same proportions, cardiolipin-like, phosphatidyl serine, phosphatidyl ethanolamine, inositol phosphatide, lecithin, and sphingomyelin. Other phosphatides were present in all the peaks.

^* Aided in part by Grant CA 06107-02 from the National Cancer Institute.

Received 10/ 4/63.