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Incidence of Mouse Bladder Tumors Following Implantation of Paraffin Pellets Containing Certain Tryptophan Metabolites^*

George T. Bryan, R. R. Brown and J. M. Price

( Division of Clinical Oncology, University of Wisconsin Medical School, Madison, Wisconsin)

Pellets of paraffin wax implanted into the lumen of the bladder of 38 mice induced one carcinoma. An equally low incidence of carcinomas was induced with paraffin wax pellets containing 15–20 per cent suspensions of 8-hydroxyquinoline or quinaldic acid, 8-hydroxyquinaldic acid, xanthurenic acid, the 8-methyl ether of xanthurenic acid, 4,8-quinolinediol, L-kynurenine sulfate, 3-hydroxy-L-kynurenine, and 3-hydroxyanthranilic acid, all metabolites of tryptophan. The inability to produce pellets of uniform composition containing these test substances was demonstrated. Paper chromatographic methods revealed that most of these compounds remained in the paraffin vehicle in vivo for as long as 342 days. It was concluded that paraffin is not a satisfactory vehicle in which to test these urinary tryptophan metabolites for bladder carcinogenicity.

^* Supported in part by Grant Nos. E-115A, E-202B, and E-116E from the American Cancer Society; by Grant Nos. A-1127 and A-1499 from the National Institutes of Arthritis and Metabolic Diseases; and by Grant Nos. C-3274 and CF-8245 from the National Cancer Institute, United States Public Health Service; and by the Elsa U. Pardee Foundation.

These results are taken from a thesis submitted by Dr. George T. Bryan to the University of Wisconsin in partial fullfillment of the requirements for the degree of Doctor of Philosophy.

Part of this investigation was carried out during the tenure of a Special Fellowship from the National Cancer Institute, United States Public Health Service.

Supported by Research Career Development Award (No. CA-K3-18,404) of the National Cancer Institute, United States Public Health Service.

American Cancer Society-Charles S. Hayden Foundation Professor of Surgery in Cancer Research.

Received 8/ 6/63.

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