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Antigenic Differences between Normal and Polyoma Virus-transformed Cells

II. In Vitro Evidence for a Virus-induced Antigen^*

Robert Bases

( California Institute of Technology, Division of Biology, Pasadena, California)

Polyoma virus-transformed hamster and mouse cells were sensitized to the killing action of complement by rabbit antisera against the transformed cells. These antisera had been previously absorbed on cells of nonpolyoma origin. The effect of such treatment was quantitated by measuring the loss of colony-forming capacity of single cells in vitro. Cells of polyoma origin were the most susceptible of those tested. One absorbed serum sensitized a polyoma-transformed mouse cell line, a hybrid cell line derived from it, but not the other parent of nonpolyoma origin. These data from in vitro experiments support the theory that transformation by polyoma virus leads to the appearance of a new cellular antigen as proposed by others on the basis of in vivo studies.

^* This investigation was supported by grants from the National Institutes of Health, U.S. Public Health Service (E1531); The National Foundation (CRBS-133); and a special fellowship (CF-13,275) from the National Cancer Institute, U.S. Public Health Service.

Present address: Service de Biologie Moleculaire, Institut Gustave Roussy, Villejuif (Seine), France.

Received 2/13/64.

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