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Microbiological Evaluation of 1,3-Bis (2-chloroethyl)-I-nitrosourea^*

Robert F. Pittillo, Annie Jo Narkates and James Burns

( Kettering-Meyer Laboratory, Affiliated with Sloan-Kettering Institute, Southern Research Institute, Birmingham, Alabama)

A new anti-leukemic agent, 1,3-bis (2-chloroethyl)-1-nitrosourea (NSC-409962), has been found to be a potent inhibitor of nonproliferating microorganisms. Comparative studies with 1-methyl-1-nitrosourea (NSC-23909) and 1-methyl-3-nitro-1-nitrosoguanidine (NSC-9369) strongly suggest a similarity in mechanism of action. A strain of Escherichia coli resistant to NSC-409962 is cross-resistant to mitomycin C, porfiromycin, several alkylating agents, azaserine, 6-diazo-5-oxo-L-norleucine, a terephthalanilide (NSC-60339), ionizing and ultraviolet irradiation, but not to 6-mercaptopurine or amethopterin. Strains of E. coli resistant to mitomycin C, porfiromycin, nitrogen mustard, or azaserine were cross-resistant to NSC-409962.

^* Supported by the Cancer Chemotherapy National Service Center, National Cancer Institute, under National Institutes of Health contract number SA-43-ph-3784.

Deceased January 3, 1964.

Received 2/17/64.