This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hampton, E. G. Search for Related Content PubMed PubMed Citation Articles by Hampton, E. G.

Viral Antigen in Rat Embryo in Culture Infected with the H-1 Virus Isolated from Transplantable Human Tumors: Cytochemical Studies^*

E. Gambetta Hampton

( Section of Virus Studies, Sloan Kettering Institute, New York, New York)

The infection of rat embryo cells with H-1 virus was followed, with Coon's method used for staining with fluorescent antibodies. After a period of 12 hours the stain revealed a diffuse fluorescence that occupied the nucleus except for areas corresponding to the nucleoli. The stained nuclei increased in number during the subsequent 16, 20, and 24 hours corresponding to the log phase of viral growth. On the 2d day after inoculation, when the viral growth curve had reached a plateau, a homogeneous type of fluorescent antibody stain was present in nuclei. Some cells contained granular or diffuse stain in the cytoplasm. At this stage acridine stain showed nuclear structural changes and homogeneous nuclei of DNA-like material. Treatment with pepsin and nucleases indicated the presence in the homogeneous nuclei of a deoxyribonucleoprotein resistant to DNase. Phase microscopy also showed nuclear alteration. By the 3d day, when the viral antigen was present in most of the cells, inclusion bodies and cytopathic effect were present. These changes were followed by the release of hemagglutinating virus.

^* This investigation was supported by Grant C-1355 from the United States Public Health Service.

Present address: Department of Biochemistry, University of Alberta, Edmonton, Canada.

Received 3/ 2/64.