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The Isolation and Cytology of Two Pigment Cell Strains from B16 Mouse Melanomas^*

( Department of Dermatology, Henry Ford Hospital, Detroit, Michigan)

Two cell lines (HFH-14, HFH-18) isolated from B16 mouse melanomas were established as monolayer cultures. These cells retained their capacity of melanin pigment formation after repeated passages in vitro. According to their morphology and developmental history, various cell types, namely, melanoblasts, melanocytes, and mature melanocytes, are identified. These cells when inoculated into C57BL mice produced typical melanotic tumors which closely resembled the original melanomas from which the cell strains were derived. These cells have all the characteristics of melanocytes and are considered to be true pigment cell strains.

^* This study was supported by a grant CA-03345 from the National Cancer Institute, U. S. Public Health Service.

Received 5/13/64.