This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sinkovics, J. G. Articles by Howe, C. D. Search for Related Content PubMed PubMed Citation Articles by Sinkovics, J. G. Articles by Howe, C. D.

Some Properties of the Photodynamically Inactivated Rauscher Mouse Leukemia Virus¹

(The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas)

Methylene blue, diluted 10^-5.0/gm weight and illuminated with visible light, inactivated the Rauscher mouse leukemia virus at 37°C and pH 7.0–7.2 in 20 min. In the dark, the virus did not bind the dye irreversibly, because virus infectivity could be retained by removing the dye from the mixture with a resin before the application of light. Resin treatment applied after the inactivation of the virus failed to reactivate viral infectivity. Mice immunized with photodynamically inactivated virus produced virus neutralizing antibodies, and spleen cells from these mice conferred antiviral immunity on recipient mice of the same line.

Vesicular stomatitis and pseudorabies viruses showed similar inactivation patterns with retention of antigenic potency.

¹ This investigation was supported by USPHS research grants CA-6529 and CA-7923 and by award 5-K3-CA through the National Cancer Institute.

Received 11/23/64.