This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kay, E. R. M. Search for Related Content PubMed PubMed Citation Articles by Kay, E. R. M.

The Effects of Tween 80 on the in Vitro Metabolism of Cells of the Ehrlich-Lettré Ascites Carcinoma¹

( Department of Biochemistry, University of Rochester, School of Medicine and Dentistry, Rochester, New York)

Marked alterations in cell permeability were brought about by the presence of the detergent Tween 80 in preparations of Ehrlich-Lettré ascites cells. The growth of these cells was normal in host mice after treatment and thus they were viable as transplants. The permeability of the cells increased greatly, as shown by the uptake of Lissamine green. Soluble nucleotides and amino acids were removed by the Tween treatment. Oxygen uptake was reduced by 50%. The incorporation of formate into protein and deoxyribonucleic acid (DNA) decreased, but it increased into nuclear ribonucleic acid (RNA). Incorporation of ³²P into the phospholipids increased greatly and was found to be localized in phosphatidyl serine. The interpretation of the alterations brought about by Tween 80 are discussed in relation to the special properties of the tumor cells.

¹ Supported by grant No. CA 05172 from the National Cancer Institute, USPHS.

Received 10/28/64.